Journal: Microfluidics and Nanofluidics

Article Title: Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics

doi: 10.1007/s10404-021-02485-0

Figure Lengend Snippet: Hydrodynamic trapping: A schematics of hydrodynamic trapping channel (Tan and Takeuchi ), B schematics of single-cell capturing and releasing (Zhang et al. ), C-1 optical micrographs showing the sequential single-cell processing in hydrodynamic channels, C-2 microfluidic chip for parallel single-cell processing based on hydrodynamic trapping (Zhang et al. )

Article Snippet: And on the integrated fluidics circuit (IFC) microfluidic chip, different kinds of reagents can be stored and injected into the center reaction unit for 96 single-cell isolation and RT-PCR amplification.

Techniques:

Journal: Microfluidics and Nanofluidics

Article Title: Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics

doi: 10.1007/s10404-021-02485-0

Figure Lengend Snippet: Single-cell nucleic acid analysis on valve-based platform: A-1 schematic of membrane-based valve, A-2 schematic of single-cell nucleic acid analysis with valve-based microfluidic chip, B integrated chip for high throughput analysis with intensively integrated valves, reactors, and channels, C fluidic control on valve-based platform (Thorsen et al. ), D different working mechanisms of membrane-based valve (Li et al. ; Sun et al. ), E integrated Fluidigm C1 system based on the concept of valve control (Pollen et al. )

Article Snippet: And on the integrated fluidics circuit (IFC) microfluidic chip, different kinds of reagents can be stored and injected into the center reaction unit for 96 single-cell isolation and RT-PCR amplification.

Techniques: Membrane, High Throughput Screening Assay, Control

Journal: Microfluidics and Nanofluidics

Article Title: Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics

doi: 10.1007/s10404-021-02485-0

Figure Lengend Snippet: Other platforms for single-cell nucleic acid analysis: A centrifugal disk chip for single-cell gene expression analysis (Furutani et al. ), B digital microfluidic chip for whole genome sequencing (Ruan et al. )

Article Snippet: And on the integrated fluidics circuit (IFC) microfluidic chip, different kinds of reagents can be stored and injected into the center reaction unit for 96 single-cell isolation and RT-PCR amplification.

Techniques: Gene Expression, Sequencing

Journal: Microfluidics and Nanofluidics

Article Title: Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics

doi: 10.1007/s10404-021-02485-0

Figure Lengend Snippet: Comparison of different microfluidic platforms for single-cell nucleic acid analysis

Article Snippet: And on the integrated fluidics circuit (IFC) microfluidic chip, different kinds of reagents can be stored and injected into the center reaction unit for 96 single-cell isolation and RT-PCR amplification.

Techniques: Comparison, Functional Assay, High Throughput Screening Assay, Control

Journal: Current Biology

Article Title: Generation of Single-Cell Transcript Variability by Repression

doi: 10.1016/j.cub.2017.05.028

Figure Lengend Snippet:

Article Snippet: Cell separation- C1 Integrated Fluidic Circuit chips , Fluidigm , 100/5759, 100/5760.

Techniques: cDNA Synthesis, Sample Prep, Marker, Software